: It calculates the expected number of alleles if all your sample sizes were equal to the smallest sample in your study.
: It allows you to compare the genetic diversity of a small population (e.g., 10 individuals) with a large one (e.g., 100 individuals) without bias [25]. 2. Required Tools and Setup na.rar
) is the number of alleles per locus in a population. However, because : It calculates the expected number of alleles
: Use the rarefaction formula to estimate the number of alleles at that standardized sample size: na.rar
: The most common software for rarefaction. It uses the Kalinowski (2005) method to calculate allelic richness [25].