Come_give_me_your_hand -

Wash your hands, put on your nightgown; look not so pale.—I tell you yet again, Banquo's buried; he cannot come out on's grave. Doctor: Even so?

This line highlights Lady Macbeth's transition from a cold, calculating figure to one "unhinged" by trauma and guilt. come_give_me_your_hand

In this scene, Lady Macbeth is mentally unraveling. While sleepwalking, she obsessively tries to wash imaginary blood from her hands and speaks to her absent husband, Macbeth. Wash your hands, put on your nightgown; look not so pale

This dramatic moment is often used in acting classes and literature studies to show "character development through breakdown". In this scene, Lady Macbeth is mentally unraveling

For your reference, here is the immediate draft of that section from Shakespeare's Macbeth :

To bed, to bed! there's knocking at the gate: come, come, come, come, give me your hand. What's done cannot be undone.—To bed, to bed, to bed! Analysis and Modern Usage