Ancient Dna: Methods And Protocols -

Synthetic DNA "adapters" are attached to the ends of the fragments, allowing them to bind to the sequencing platform.

Proteinase K is added to break down cellular proteins and nucleases.

Over time, DNA strands break into very short fragments, typically between 30 and 100 base pairs.

Success begins with choosing the right material. The (part of the skull) and tooth cementum are the "gold standards" because their high density protects DNA from environmental leaching.

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